Overview:

PureScript II Pro Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H ) Reverse Transcriptase. Compared with the previous generation reverse transcriptase, the thermo-stability of this product is significantly improved. The half-life of PureScript II Pro Reverse Transcriptase at 50°C is >240 min. At 55°C, the half-life of the enzyme is >1 hour, which significantly benefits the transcription of RNA templates with complex secondary structure. In addition, it has an improved template affinity and cDNA synthesis efficiency. It has a good resistance to most RT-PCR inhibitors and is suitable for long-fragment cDNA amplification (up to 20 kb). PureScript II Pro Reverse Transcriptase is applicable for reverse transcription of animal, microbial, and plant RNA rich in polysaccharides and polyphenols.

References:

1. Kotewicz ML, D'Alessio JM, Driftmier KM, Blodgett KP, Gerard GF. Cloning and overexpression of Moloney murine leukemia virus reverse transcriptase in Escherichia coli. Gene. 1985;35(3):249-58. doi: 10.1016/0378-1119(85)90003-4. PMID: 2412939.

Storage and Stability:

Store product at –30oC to –15oC. For optimal performance aliquot product into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated handling and multiple freeze/thaw cycles.