Recent Publications Featuring SignalChem Diagnostics CRISPR Products

SignalChem Diagnostics develops recombinant CRISPR proteins and molecular biology reagents that support assay development across molecular diagnostics, biosensing, synthetic biology, and life science research. The publications highlighted below represent a selection of recent studies that incorporated our products into a variety of workflows.

LwCas13a

Our LwCas13a protein has been featured in several recent publications spanning molecular diagnostics, biosensor development, and synthetic biology.

In a 2025 study published in Nature Communications, researchers developed a room-temperature RNA detection platform based on CRISPR anti-tag regulation of Cas13a activity, demonstrating a simplified approach for sensitive RNA detection.

Researchers publishing in Biosensors and Bioelectronics (2026) developed CLAMP, a PCR-free cascaded CRISPR-Cas13a colorimetric assay for non-invasive microRNA-based subtyping of allergic rhinitis, using LwCas13a as the detection enzyme.

A 2026 study in Bioelectrochemistry integrated LwCas13a into an electrochemiluminescence sensing platform that combined CRISPR-mediated target recognition with perovskite-enabled electrochemiluminescent signal generation for highly sensitive nucleic acid detection.

LwCas13a was also incorporated into a novel SHERLOCK assay for the clinical detection of Mycoplasma genitalium, published in Microbiology Spectrum (2025), where the authors evaluated the assay using clinical samples.

Beyond molecular diagnostics, two preprints published on ChemRxiv (2025 and 2026) used LwCas13a in synthetic biomolecular systems that converted DNA-binding proteins and transcription factors into programmable inputs for CRISPR-based molecular networks.

Selected publications

• Moon J. et al. (2025). Nature Communications. CRISPR anti-tag-mediated room-temperature RNA detection using CRISPR/Cas13a
• Wan Y. et al. (2026). Biosensors and Bioelectronics. PCR-free cascaded CRISPR-Cas13a colorimetric platform (CLAMP) for non-invasive miRNA-based allergen-specific subtyping of allergic rhinitis
• Xie K. et al. (2026). Bioelectrochemistry. Silica-detoxified perovskite ECL: Cas13a-triggered signal-on sensing with CsPbBr₃@SiO₂@Au
• Omachi R. et al. (2025). Microbiology Spectrum. Development and clinical evaluation of a novel SHERLOCK test for Mycoplasma genitalium
• Capelli L. et al. (2025). ChemRxiv. Structure-Switching DNA Translators for the Integration of Transcription Factor-Driven CRISPR-Cas12a Activity into Synthetic Biomolecular Networks
• Spezzani E. et al. (2026). ChemRxiv. Repurposing DNA-Binding Proteins as Molecular Actuators in Synthetic Systems Through Proximity-Driven DNA Reactions

AapCas12b

SignalChem Diagnostics' AapCas12b protein has also been used in studies focused on infectious disease detection and veterinary diagnostics.

A 2025 publication in Frontiers in Molecular Biosciences described an ultra-sensitive CRISPR-Cas12b recombinase polymerase amplification (RPA) assay for active Trypanosoma brucei evansi infections. The methodology was later presented as a standardized laboratory protocol in Euglenozoa: Methods and Protocols (2026), providing researchers with detailed, reproducible experimental procedures.

In addition, a 2025 publication in the Journal of Animal Health and Production described a CRISPR-Cas12b-based RPA assay for detecting and genetically profiling parasitic infections in domesticated animals, focusing on Trypanosoma brucei evansi detection.

Selected publications

• Álvarez-Rodríguez A. et al. (2025). Frontiers in Molecular Biosciences. A CRISPR-Cas-based recombinase polymerase amplification assay for ultra-sensitive detection of active Trypanosoma brucei evansi infections
• Li Z. et al. (2026). Euglenozoa: Methods and Protocols. Standardized Recombinase Polymerase Amplification (RPA) Assay for Detecting Active Trypanosoma brucei evansi Infections
• Lahhob Q.R. et al. (2025). Journal of Animal Health and Production. A Revolutionary CRISPR-Based Detection and Genetic Profiling of Parasitic Infections in Domesticated Animals

LbuCas13a

In a 2024 publication in Biosensors, researchers developed an aptamer-based molecular switching system that enabled communication between two otherwise non-interacting proteins, Cas13a and T7 RNA polymerase. LbuCas13a was used to cleave RNA aptamer or blocker sequences in response to an RNA activator, thereby regulating T7 RNA polymerase activity and demonstrating a synthetic protein-communication system beyond conventional diagnostic workflows.

Selected publication

• Kim Y. et al. (2024). Biosensors. Aptamer-Based Switching System for Communication of Non-Interacting Proteins

Cas13 Reaction Buffer

SignalChem Diagnostics' Cas13 Reaction Buffer was also cited alongside our LwCas13a protein in the Microbiology Spectrum study describing a clinically evaluated SHERLOCK assay for Mycoplasma genitalium, supporting the CRISPR-Cas13 detection workflow.

Selected publication

• Omachi R. et al. (2025). Microbiology Spectrum. Development and clinical evaluation of a novel SHERLOCK test for Mycoplasma genitalium

Cas12 ssDNA Reporter

Our Cas12 ssDNA Reporter was featured in a 2026 study published in Frontiers in Microbiology, where researchers developed a rapid RPA-CRISPR/Cas12a assay for detection of Rickettsia rickettsii. The reporter molecule enabled fluorescence-based signal generation following Cas12a activation.

Selected publication

• Ozubek S. et al. (2026). Frontiers in Microbiology. A rapid isothermal RPA-CRISPR/Cas12a assay for detection of Rickettsia rickettsii

Looking Ahead

The publications highlighted here illustrate the broad range of applications supported by SignalChem Diagnostics CRISPR products, from infectious disease diagnostics and biosensing to synthetic biology and molecular engineering. As researchers continue to develop new CRISPR-enabled technologies, we remain committed to providing high-quality recombinant proteins and molecular biology reagents that support innovation across academic, clinical, and industrial research.