Bsu DNA Polymerase Large Fragment

Overview:

DNA polymerase (DNAP) is an enzyme that catalyzes the DNA molecules and is crucial for DNA replication. Bsu DNA polymerase large fragment is a product of the Bacillus subtilis DNA polymerase I that retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ and 3´→ 5´ exonuclease activity (1). Bsu DNA polymerase has a wide range of applications such as primer labelling, second-strand cDNA synthesis, DNA strand displacement synthesis, recombinase polymerase amplification, and single dA tailing (2).

Gene Aliases:

polA, DNA polymerase I

Genebank Number:

AF008220
AAC00350.1

Formulation:

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 20% glycerol, 1 mM DTT.

References:

1. Okazaki, T. et al. J. Biol.Chem. 1964, 239: 259-268. 2. Piepenburg, O. et al. PLOSBiology. 2006, 4: 1115-1121.

Storage and Stability:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity:

5 U/µL