AapCas12b

Alternate Names:

c2c1, CRISPR-associated endonuclease C2c1, Cas12b

Overview:

CRISPR (clustered regularly interspaced short palindromic repeats) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been leveraged to create an exemplary genome editing tool with applications in both basic research and therapeutics development (3). AapCas12b is a type V-B CRISPR-Cas effector protein. Due to its thermostability, can be used in a wide range of biomedical applications including loop-mediated isothermal amplification (LAMP), mammalian genome editing, and gene activation (4).

Accession Number:

WP_067623834.1

Formulation:

Protein stored in 25 mM Tris, 300 mM NaCl, pH7.5, 50% glycerol, 0.1 mM TCEP

References:

: 1. Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
3. Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.
4. Teng F, et al. 2018. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell discovery. 4(1):1-5.

Storage and Stability:

Store at –20°C. To avoid repeated handling and multiple freeze/thaw cycles, aliquot product into smaller quantities.

Concentration:

1.0 µg/µl
7.66 pmol/µg

Purity:

>80%

Molecular weight:

Calculated MW: 130.5 kDa
Observed MW: ~128 kDa

Recommendation:

Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.