2019-nCoV Nucleocapsid (N) Protein

Overview:

CoV N-protein is an RNA-binding protein primarily involved in binding to the viral RNA genome during packaging of viral RNA into a helical ribonucleoproteins or nucelocapsid structure (1). In addition, N-protein regulates viral RNA synthesis during replication/transcription and modulates host-pathogen interactions, such as such as actin reorganization, host cell cycle progression, and apoptosis (2). N-protein is abundantly expressed during infection and antibodies against N-protein have been detected in confirmed cases of Covid-19. Therefore, N-protein is a potent antigen that provides basis for future vaccine and diagnostic kit development (3).

Gene Aliases:

Nucleoprotein, Nucleocapsid protein, NC, Protein N.

Genebank Number:

QHD43423

Formulation:

10mM PBS, pH 8.0, 50mM NaCl, 0.05% Na3N.

References:

1. McBride R, et al: The coronavirus nucleocapsid is a multifunctional protein. Viruses. 2014, 6:2991-3018.

2. Surjit M, et al: The nucleocapsid protein of severe acute respiratory syndrome-coronavirus inhibits the activity of cyclin-cyclin-dependent kinase complex and blocks S phase progression in mammalian cells. J Biol Chem. 2006, 281:10669-10681

3. Zeng W, et al: Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochem Biophys Res Commun. 2020, 527(3):618‐623.

Specific Activity:

Binding of Anti-2019-nCoV N-Protein Antibody (C19NP-60H) to immobilized 2019-nCoV Nucleocapsid (N) Protein (C19NP-G242H) determined by ELISA using Goat anti-human Fc-HRP.

Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping:

Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight:

52 kDa