2019-nCoV Spike RBD-N Protein Chimera

Overview:

The severe acute respiratory syndrome-related novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world in 2020 (1). The spike glycoprotein (S) of coronavirus belongs to the type I transmembrane protein containing two subunits, S1 and S2 (2), which is also known to be the key component to bind with host cells through the interaction with angiotensin-converting enzyme 2 (ACE2) (3). N-protein is a potent antigen that provides the basis for future vaccine and diagnostic kit development (4).

Gene Aliases:

2019-nCoV S1, SARS-CoV-2 spike S1, SARS-CoV-2 S1, novel coronavirus spike s1, nCov spike s1, coronavirus spike S1; Nucleoprotein, Nucleocapsid protein, NC, Protein N

Genebank Number:

RBD MN908947; N protein QHD43423
QHD43423

Formulation:

Recombinant protein stored in 1X PBS, pH 7.4

References:

1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3. Lan J, et al: Crystal structure of the 2019-nCov spike receptor-binding domain bound with the ACE2 receptor. bioRxiv. doi: https://doi.org/10.1101/2020.02.19.956235.

4. McBride R, et al: The coronavirus nucleocapsid is a multifunc-tional protein. Viruses. 2014, 6:2991-3018.

Specific Activity:

Sample Activity Plot. For specific information on a given lot, see the related technical data sheet.

Molecular Weight:

Determined to be approx. MW, 122 kDa.

Purity:

Determined to be >70% by SDS-PAGE quantitative densitometry.

Storage, Stability, and Shipping:

Store product at –70^oC. For optimal storage, aliquot targets into smaller quantities after centrifugation and store at the recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.