2019-nCoV PLpro, Active

Overview:

2019-nCoV papain-like protease (PLpro), also known as SARS-CoV-2 PLpro, is a virally encoded cysteine protease that acts on three cleavage sites of the viral polyprotein to release mature non-structural proteins (nsp) 1, 2 and 3, a process that is essential for viral replication. In addition, PLpro possesses deubiquitinating and deISGylating activities, thus negatively regulating the ubiquitin and ISG15-dependent host immune response during viral infection. PLpro also participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.

Gene Aliases:

SARS-CoV-2 PLpro

Genebank Number:

QHD43415

Formulation:

50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol.

References:

1. Wu F. et al: A new coronavirus associated with human respiratory disease in China. Nature 579: 265-269, 2020

2. Báez-Santos Y. M. et al: The SARS-coronavirus papain-like protease: structure, function and inhibition by designed antiviral compounds. Antiviral Res. 115: 21-38, 2015

3. Lindner H. A. et al: The papain-like protease from the severe acute respiratory syndrome coronavirus is a deubiquitinating enzyme. J Virol. 79:15199-15208, 2005

Activity:

PLpro inhibition profile in the presence of various concentrations of PR-619, a broad spectrum and reversible DUB inhibitor.

Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping:

Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications:

Molecular Weight:

38 kDa