1st Strand cDNA Synthesis SuperMix for qPCR (gDNA Digester Plus)

Specifications:

Type of end product: cDNA (first-strand)
PCR method: RT-PCR
Type of reagent: Reverse Transcription
Type of sample: RNA
Optimal reaction temperature: 55°C-60°C
Reverse transcriptase: M-MLV

Overview:

SignalChem Diagnostics 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA Digester Plus) is a ready-to-use premix that includes our Reverse Transcriptase, High-Yield, Fast, and Thermostable (Cat# RTH04-E29B). This reverse transcriptase offers high thermostability, tolerating reaction temperatures up to 60°C, and can be used for reverse transcription of RNA templates with complex secondary structures. Additionally, the enzyme has enhanced template affinity, making it particularly effective for transcribing low-copy targets.

The premix includes 5X gDNA Digester Mix and 4X SuperMix Plus. The 5X gDNA Digester Mix removes residual genomic DNA contamination in RNA templates, ensuring more reliable downstream results. The 4X SuperMix Plus contains all the essential components for reverse transcription, including buffer, dNTPs, Reverse Transcriptase (Cat# RTH04-E29B), RNase inhibitor, and a proportionally optimized Random Primers/Oligo (dT)18 Primer Mix. Add your RNA template and RNase-free water to initiate reverse transcription.

This product is designed for two-step RT-qPCR and is specifically optimized for qPCR applications. Proportionally optimized Random Primers/Oligo (dT)18 Primer Mix ensures uniform reverse transcription efficiency across all regions of the RNA transcript, maximizing the accuracy and reproducibility of qPCR results. The resulting cDNA is compatible with both the SYBR Green and TaqMan probe qPCR methods.

Components:

RNase-free water: 1 mL (for 10T); 2 x 1 mL (for 100T)
5X gDNA digester Mix: 30 μL (for 10T); 300 μL (for 100T)
4X SuperMix Plus: 50 μL (for 10T); 500 μL (for 100T)

Storage and Shipping:

Store at -25°C ~ -15°C for up to 18 months.