FnCas12a, Glycerol-Free

Alternate Names:

CRISPR-associated endonuclease Cas12a, CRISPR-associated endonuclease Cpf1 1, FnCas12a 1, FnCpf1

UniProt Number:

A0Q7Q2

Overview:

CRISPR (clustered regularly interspaced short palindromic repeat) and CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for RNA-based therapeutics development (3). Cas12 and Cas13 have collateral nucleotide cleavage activities and are widely used in CRISPR-based diagnostic platforms. FnCas12a from Francisella shows increased stability at >37◦C making it ideal for LAMP (loop-mediated isothermal amplification) (4).

Accession Number:

WP_003040289

Formulation:

Protein stored in 25mM Tris, 300mM NaCl pH7.5 0.1mM TCEP

References:

1. Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
3. Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.
4. Mohanraju P, et al: Development of a Cas12a-Based Genome Editing Tool for Moderate Thermophiles. CRISPR J. Feb;4(1):82-91. 2021.

Storage and Stability:

Store at –70°C. To avoid repeated handling and multiple freeze/thaw cycles aliquot diluted product into smaller quantities.

Concentration:

1.0 μg/μl
6.53 pmol/μg

Molecular Weight:

Calculated: 153.2 kDa
Observed: ~130 kDa

Purity:

>90%