TtCsm6

Recombinant Thermus thermophilus protein TtCsm6 was expressed in E. coli cells using a C-terminal His tag.

Catalog No.	CS06-E311H CS06-E311H-100
Size	50 µg100 µg Bulk Customize
Quantity
Price	$ 235

Catalog No.	Pack Size	Price (USD)
CS06-E311H-50	50 µg	$140
CS06-E311H-100	100 µg	$235
CS06-E311H-BULK	BULK	Contact Us

PRODUCT OVERVIEW
PRODUCT DATASHEETS

Alternate Names:

CRISPR system endoribronuclease Csm6, CRISPR type III-A associated protein Csm6

UniProt Number:

Q53W17

Overview:

TtCsm6 is an endoribonuclease specific for single-stranded RNA that consists of the N-terminal CARF domain and the C-terminal HEPN domain. The ribonuclease active site is formed by the HEPN domain dimer. TtCsm6 belongs to the Class 1 type-III-A CRISPR-Cas system (1). Upon activation of the CRISPR ancillary ribonucleases Csx1/Csm6, the HEPN active site degrades invasive RNA non-specifically. TtCsm6 is activated by the cyclic oligoadenylate second messengers that was converted from ATP by the Cas10 subunit of the Type III interference complex (2).

Accession Number:

WP_011229148

Formulation:

Protein stored in 50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 150 mM imidazole, 1 mM DTT, 10% glycerol

References:

1. Niewoehner, O. et al. 2016. Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. RNA 22(3):318-329.
2. Niewoehner, O. et al. 2017. Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548.

Storage and Stability:

Store at –70°C. To avoid repeated handling and multiple freeze/thaw cycles aliquot product into smaller quantities.

Concentration:

1.0 μg/μl
18.18 pmol/μg

Purity:

>90%

Molecular Weight:

Calculated: 52.7 kDa
Observed: ~50 kDa

Recommendation:

Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.

CS06-E311H-TtCsm6.pdf

SDS-CS06-E311H-TtCsm6.pdf

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