LbuCas13a

Alternate Names:

CRISPR-associated endoribonuclease C2c2, EndoRNase, LbuC2c2, c2c2, Cas13a

UniProt Number:

C7NBY4

Overview:

Cas13a is an RNA-guided RNA endonuclease that belongs to the class 2 type VI CRISPR-Cas system (1). Cas13a possesses both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains (2). Interestingly, activated Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner (3). Engineered LbuCas13a shows stability and altered cleavage activity in vitro and possesses the capability of robust exon skipping in human cells (4).

Accession Number:

WP_015770004.1

Formulation:

Protein stored in 50 mM Tris-HCL, pH 7.5, 600 mM NaCl, 2 mM DTT, 10% glycerol

References:

1. East-Seletsky A, et al. 2016. Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 538:270–3.
2. Abudayyeh O, et al. 2016. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 353:5573.
3. Zhang J, You Y. 2020. CRISPR-Cas13a system: A novel approach to precision oncology. Cancer Biol Med. 17(1):6-8.
4. Patchsung M, et al. 2020. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 4(12):1140-1149.

Storage and Stability:

Store at –70°C. To avoid repeated handling and multiple freeze/thaw cycles aliquot diluted product into smaller quantities.

Concentration:

0.2 μg/μl
7.14 pmol/μg

Recommendation:

Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions

Purity:

>90%

Molecular Weight:

Calculated: 138.5 kDa
Observed: ~120 kDa