CcaCas13b

Alternate Names:

Type VI-B CRISPR-associated RNA-guided ribonuclease Cas13b, Cas13b

Overview:

CRISPR-Cas systems constitute the adaptive immune system in bacteria via RNA-guided endonucleases (1,2). This system has been leveraged to create an exemplary genome editing tool with applications in both basic research and therapeutics development (3). CcaCas13b is an effector protein that belongs to the Class 2 subtype VI-B CRISPR-Cas system (3). It processes its own CRISPR array with short and long direct repeats, cleaves target RNA, and exhibits collateral RNase activity (3). It has been shown to effectively target and silence distinct mammalian ssRNA viruses (4).

Accession Number:

WP_013997271

Formulation:

Protein stored in 50 mM Tris, pH 7.5, 600 mM NaCl, 0.1 mM TCEP, and 50% glycerol

References:

1. Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
3. Smargon, A. A., et al. 2017. Cas13b is a Type VI-B CRISPR-associated RNA-Guided RNAse differentially regulated by accessory proteins Csx27 and Csx28. Mol. Cell. 65(4):618-630.
4. Freije, C. A., et al. 2019. Programmable Inhibition and Detection of RNA Viruses Using Cas13. Mol. Cell. 76(5):826-837.

Storage and Stability:

Store at –20°C. To avoid repeated handling and multiple freeze/thaw cycles aliquot product into smaller quantities.

Concentration:

1.0 μg/μl
6.9 pmol/μg

Molecular Weight:

Calculated: 144.7 kDa
Observed: ~130 kDa

Purity:

>90%

Recommendation:

Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.