Recombinant endonuclease that digests both single-stranded and double-stranded DNA, used to remove DNA contaminants from RNA and protein samples for optimal purity in downstream applications.
Expression Host: E. coli
Optimum Temperature: 37°C
Storage Buffer: 10 mM Tris-HCl pH 7.6, 2 mM CaCl2, 50% (v/v) Glycerol
Unit Definition: The amount of enzyme needed to completely degrade 1 μg of plasmid DNA in 10 minutes at 37°C. Reaction buffer: 10 mM Tris-HCl pH 7.6, 2.5 mM MgCl2, 0.5 mM CaCl2, 1 μg plasmid DNA
Overview:
SignalChem Diagnostics DNase I (Deoxyribonuclease I), GMP-Grade (2 U/μL) is a high-purity, recombinant enzyme designed to degrade and remove DNA contaminants from RNA and protein samples, ensuring optimal purity for downstream applications. Provided in the aqueous form, it is an endonuclease that digests both single-stranded and double-stranded DNA, breaking them down into single deoxynucleotides or short single- or double-stranded oligodeoxynucleotides. DNase I hydrolyzes phosphodiester bonds, generating monodeoxynucleotides and oligodeoxynucleotides with 5'-phosphate and 3'-OH groups. The smallest digestion product is typically a poly-tetranucleotide.
DNase I can act on various DNA forms, including single-stranded DNA, double-stranded DNA, and chromatin, though its cutting efficiency in chromatin is influenced by histones. It functions optimally at pH 7-8 and requires Ca²⁺ for activity. The enzyme can be activated by divalent metal ions such as Co²⁺, Mn²⁺, and Zn²⁺. 5 mM Ca²⁺ can protect the enzyme from hydrolysis.
In the presence of Mg²⁺, DNase I cleaves DNA randomly at any site on either strand. However, in the presence of Mn²⁺, it cuts both strands at nearly the same location, producing either blunt ends or sticky ends with 1-2 nucleotide overhangs.
DNase I is commonly used to remove template DNA after in vitro transcription and ensure DNA-free RNA for RT-PCR and RT-qPCR. It is also used in DNA labeling via nick translation in combination with DNA polymerase I, as well as in DNA fragmentation for library construction.
The robust performance and compliance with GMP standards of this product make it a reliable choice for high-quality molecular biology and biopharmaceutical applications.
Storage and Shipping:
Ship on dry ice and store at -25°C ~ -15°C for up to 1 year.
