DNase I, RNase-Free

Specifications:

Expression Host: E. coli
Unit Definition: One activity unit (Kunitz Unit) is defined as the amount of enzyme that causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C and pH 5.0, using calf thymus DNA as the substrate.

Overview:

SignalChem Diagnostics DNase I (RNase-Free) is an endonuclease that degrades both single-stranded and double-stranded DNA. It hydrolyzes phosphodiester bonds, generating mono- and oligodeoxynucleotides with 5'-phosphate and 3'-hydroxyl groups. DNase I requires Ca²⁺ and has optimal activity at pH 7-8. DNase I can be activated by divalent metal ions such as Co²⁺, Mn²⁺, and Zn²⁺. In the presence of Mg²⁺, DNase I sporadically cleaves individual strands in double-stranded DNA. in the presence of Mn²⁺, it cleaves both strands at the same site, producing either blunt ends or sticky ends with 1–2-nucleotide overhangs. This product can be used for RNA sample processing.

Components:

DNase I, RNase-Free (2 U/μL): 500 μL (for 1,000 U); 5 x 500 μL (for 5,000 U)
DNase I Reaction Buffer (10X): 1 mL (for 1,000 U); 5 x 1 mL(for 5,000 U)

Storage and Shipping:

Store at -25°C ~ -15°C for up to two years. Avoid repeated freeze-thaw cycles.