Dual-Mode RNA Library Prep Kit

Specifications:

Library type: RNA library
Strand-specificity: dNTP/dUTP
Total RNA input amount: 10 ng-4 μg
Library preparation total time: ~5h
Instrument compatibility: Illumina or MGI Platforms
Recommended application: Gene expression

Overview:

SignalChem Diagnostics Dual-Mode RNA Library Prep Kit is a comprehensive solution for total RNA sequencing library construction, compatible with both Illumina™ and MGI™ platforms. It includes reagents for RNA fragmentation, reverse transcription, conventional and strand-specific double-stranded cDNA synthesis, and library amplification.

Library construction can be performed following mRNA purification or rRNA depletion. The two-strand synthesis module features two distinct buffers to support both conventional and strand-specific library preparation. In the strand-specific buffer, dTTP is replaced with dUTP, allowing dUTP incorporation into the second cDNA strand. Since the high-fidelity DNA polymerase used in this kit cannot amplify uracil-containing DNA, strand specificity is ensured.

Components:

2X Frag/Prime Buffer: 80 μL (for 8 T); 250 μL (for 24 T); 930 μL (for 96 T)
1st Strand Enzyme Mix: 16 μL (for 8 T); 48 μL (for 24 T); 192 μL (for 96 T)
Strand Specificity Reagent: 50 μL (for 8 T); 150 μL (for 24 T); 580 μL (for 96 T)
2nd Strand Buffer (dNTP): 240 μL (for 8 T); 720 μL (for 24 T); 2 x 1440 μL (for 96 T)
2nd Strand Buffer (dUTP): 240 μL (for 8 T); 720 μL (for 24 T); 2 x 1440 μL (for 96 T)
2nd Strand Enzyme Master Mix: 40 μL (for 8 T); 120 μL (for 24 T); 480 μL (for 96 T)
Ligation Enhancer: 240 μL (for 8 T); 720 μL (for 24 T); 2 x 1440 μL (for 96 T)
Novel T4 DNA Ligase: 40 μL (for 8 T); 120 μL (for 24 T); 480 μL (for 96 T)

Storage and Shipping:

Store at -25°C ~ -15°C for up to 1 year.