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Glycerol Kinase

This enzyme is a diagnostic grade reagent from a microorganism host. It is useful for enzymatic determination of glycerol and of triglyceride when coupled with glycerol-3-phosphate oxidase or pyruvate kinase and lactate dehydrogenase, lipoprotein lipase in clinical analysis.

GK01D1-E311H
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Storage and Stability:

Product can be stored at 2~8°C for transportation process up to ten days but long-term storage should be at –20°C


Activity:

≥ 30 U/mg solid


Unit Definition:

One unit causes the formation of one micromole of hydrogen peroxide (half a micromole of quinoneimine dye) per minute at pH 7.9 and 37°C


EC:

EC 2.7.1.30


Appearance:

White amorphous powder, lyophilized


Molecular Weight:

56.1 kDa


Contaminants:

Catalase ≤1.0 × 10-1 %
NADH oxidase ≤1.0 × 10-3 %
Adenosine triphosphatase ≤1.0 × 10-3 %


Isoelectric Point:

5.83


Michaelis Constant:

6.88 ×10-5 M (Glycerol), 4.88 ×10-5 M (NAD+)


Inhibitors:

Ag+, Hg2+, NEM, SDS, Proclin-300


Optimum pH:

7.5 ~ 8.0


Optimum Temperature:

50°C


pH Stability:

pH 5.5–10.0 (25°C, 20 hr)


Thermal Stability:

Below 65°C (pH 7.5, 30 min)




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