Human Transforming Growth Factor Beta 2 (TGF-β2) ELISA Kit

Overview:

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to TGF-β2. Samples are added to the microplate wells and bind the specific antibody. Then a biotinylated polyclonal antibody specific to TGF-β2 is added to each well and incubated forming an antibody-antigen complex. Following a wash to remove any unbound reagent, HRP-avidin is added to each well to bind the biotin-labeled antibody. After the unbound HRP-avidin has been washed away a color reagent is added to each well for coloration. Wells that contain TGF-β2 will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm.

Storage and Stability:

Store all reagents at 2–8°C. Product stored under these conditions should be stable for 2 weeks. For long-term storage, store all reagents at -20°C for up to 12 months from the production date

Components:

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