mRNA Cap 2’-O-Methyltransferase, GMP-Grade (50 U/μL)

Specifications:

Host: E. coli
Optimum Temperature: 37°C
Storage Buffer: 20 mM Tris-HCl pH 8.0, 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 50% (v/v) glycerin, 0.1% (v/v) Trion X-100
Unit Definition: One unit is defined as the amount of enzyme needed to methylate 10 pmol of an 80-nucleotide capped RNA transcript in 1 hour at 37°C.

Storage and Shipping:

Ship with dry ice and store at -25°C ~ -15°C for up to 1 year.

Overview:

SignalChem Diagnostics mRNA Cap 2'-O-Methyltransferase, GMP-Grade (50 U/μl) is a recombinant enzyme derived from vaccinia virus. It catalyzes the 2'-O methylation of the first nucleotide in the 5' cap structure of mRNA, using SAM as a methyl donor to form the Cap1 structure. For this, the enzyme requires RNA with m7GpppN cap structure as a substrate. The resulting Cap1 structure enhances mRNA translation efficiency, improving expression in transfection and microinjection experiments. Manufactured under GMP-compliant conditions, it is produced in the aqueous form and can be used for in vivo and in vitro Cap1 capping reactions.