Mouse Theiler's Murine Encephalomyelitis Virus (TMEV) ELISA Kit

Overview:

The microplate provided in this kit has been pre-coated with an antibody specific to TMEV. Samples are added to the microplate wells and bind the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for TMEV is added to each well and incubated forming an antibody-antigen-enzyme labeled antibody complex. Following a wash to remove any unbound reagent, TMB substrate solution is added to each well. Wells that contain TMEV and HRP conjugated TMEV antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of TMEV is determined by comparing with the CUTOFF value.

Storage and Stability:

Store all reagents at 2–8°C. Product stored under these conditions should be stable for 6 months from the production date

Components:

Components Image