Protein G

Overview:

Immunoglobulin (Ig) binding protein, Protein G, is present on the surface of group C and G Streptococcal bacteria. Protein G is known to bind the Fab and Fc regions of the antibody in the host cell and suppresses host cell immune responses (1). Such cross-linking is found to prevent opsonophagocytosis and result in B-cell death in vitro (2). All four human IgG subclasses interact with protein G with high affinity (3). Overall, protein G can be used as an immunological reagent in a broad range of studies that involve detection of antibody-secreting cells (4).

Formulation:

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 200mM imidazole, 1mM DTT, 10% glycerol.

Storage and Stability:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Alternative Name(s):

SpG, Streptococcal protein G, Immunoglobulin G-binding protein G, IgG-binding protein G

Specific Activity:

Sample activity data. for lot-specific data, please refer to the lot-specific datasheet.

Purity:

Sample purity data. For lot-specific data, please refer to the lot-specific datasheet.

Molecular weight:

~37 kDa