Recombinant enzyme that catalyzes the hydrolysis of inorganic pyrophosphate (PPi) into orthophosphate (Pi) and can be used to remove PPi and improve RNA yield. Supplied at 0.1 U/μL.
Host: expressed in E.coli
Optimum Temperature: 25°C
Storage Buffer: 50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% (v/v), TritonX-100, 50% (v/v) glycerin
Unit Definition: One unit (U) of the enzyme is defined as the amount that catalyzes the hydrolysis of pyrophosphate (PPi) to release 1 μmol of orthophosphate (Pi) per minute. The standard reaction is carried out in a 500 μL system containing 100 mM Tris-HCl (pH 7.2), 2 mM MgCl₂, and 2 mM PPi, incubated at 25°C for 10 minutes.
Overview:
SignalChem Diagnostics Inorganic Pyrophosphatase, GMP-Grade (0.1 U/μL) is a high-purity, recombinant enzyme designed to catalyze the hydrolysis of inorganic pyrophosphate (PPi) into orthophosphate (Pi), ensuring efficient removal of PPi in various molecular biology applications. It is a high-energy reaction that can be used to drive thermodynamically unfavorable processes to completion. In molecular biology, it increases RNA yield in reverse transcription reactions. Manufactured under strict Good Manufacturing Practice (GMP) guidelines, this enzyme is ideal for applications where precision, reliability, and consistency are critical.
Storage and Shipping:
Ship on dry ice and store at -25°C ~ -15°C for up to 1 year.
Additional Information:
1. This enzyme remains active in a variety of reaction buffers and can typically be used directly in applications such as in vitro transcription (IVT).
2. The optimal reaction temperature is 25°C, though the enzyme remains active between 16°C and 37°C. Heating to 65°C for 10 minutes will inactivate the enzyme.
