Appearance: Powder
Quantity: 100 mg/1 g
Product Type: RNase A
Overview:
SignalChem Diagnostics Ribonuclease A (RNase A) From Bovine Pancreas is a single-chain polypeptide with four disulfide bonds and a molecular weight of approximately 13.7 kDa. It is an endoribonuclease that specifically cleaves single-stranded RNA at pyrimidine nucleotides. Specifically, RNase A recognizes the phosphodiester bond between the 5'-ribose of one nucleotide and the phosphate group on the 3'-ribose of an adjacent pyrimidine nucleotide, hydrolyzing 2',3'-cyclic phosphates into corresponding 3'-nucleoside phosphates (e.g., pG-pG-pC-pA-pG is cleaved by RNase A to generate pG-pG-pCp and A-pG).
RNase A is most active in cleaving single-stranded RNA. The recommended working concentration is 1–100 μg/mL, making it compatible with various reaction systems. At low salt concentrations (0–100 mM NaCl), RNase A can degrade single-stranded RNA, double-stranded RNA, and RNA-DNA hybrids. However, at high salt concentrations (>0.3 M NaCl), it specifically targets single-stranded RNA.
RNase A is commonly used to remove RNA during plasmid or genomic DNA preparation. DNase activity during this process can significantly impact the reaction, and heat inactivation in a water bath can be used to eliminate DNase activity. This product is free of DNase and protease and does not require heat treatment before use. Additionally, RNase A can be used in RNase sequence analysis and protection assays.
