RNase H (5 U/μL)

Specifications:

Source: Shigella flexneri
Molecular Weight: 19.7 KDa
Concentration: 5 U/μl
Unit Definition: One unit is defined as the amount of enzyme required to hydrolyze RNA-DNA hybrids and release 1 nmol of ribonucleotides in a 50 μL reaction system within 20 minutes at 37°C.

Overview:

SignalChem Diagnostics RNase H (5 U/μL), also known as ribonuclease H, is an endoribonuclease that specifically hydrolyzes phosphodiester bonds in the RNA strand of DNA/RNA hybrids. However, it does not hydrolyze phosphodiester bonds in single-stranded or double-stranded DNA or RNA.

Components:

RNase H (5 U/μL): 20 μL OR 100 μL OR 1 mL
10X RNase H Reaction Buffer: 200 μL OR 1 mL OR 10 X 1 ml
RNase H Reaction Buffer formulation: 750 mM KCI, 500 mM Tris-HCI, 30 mM MgCl2, 100 mM Dithiothreitol, pH 8.3 at 25°C

Storage and Shipping:

Store at-25~-15°C for up to 2 years.

Applications:

1. Removal of mRNA prior to second-strand cDNA synthesis.
2. Degradation of RNA following first-strand cDNA synthesis in RT-PCR or RT-qPCR workflows.
3. Removal of poly(A) tails after hybridization of Oligo(dT) to mRNA.
4. Site-specific cleavage of RNA.