RNase H II (2 U/μL)

Storage and Stability:

Store at-25~-15°C for up to 2 years

Overview:

SignalChem Diagnostics RNase H II (2 U/μL) is an endoribonuclease derived from Pyrococcus abyssi and expressed in E. coli. It recognizes DNA/RNA duplexes and cleaves the RNA strand from the 5’ end, generating a 5’ phosphate and a 3’ hydroxyl group. However, it has minimal activity on single-stranded RNA and does not cut double-stranded or single-stranded DNA. RNase H II exhibits optimal activity at 70–75°C, remains active between 50°C and 75°C, and has very low activity at room temperature. This enzyme is highly thermostable, retaining nearly full activity even after 45 minutes at 95°C, and is compatible with various PCR reaction systems.

Components:

RNase H II (2 U/μL): 125 μL

Applications:

1. Detection using LAMP with high-sensitivity probes
2. RNase H II-dependent PCR (rhPCR)
3. Removal of mismatched ribonucleotides generated during PCR
4. Degradation of the RNA component of Okazaki fragments.