Advanced Reverse Transcriptase (200 U/µL)

Specifications:

Final product: 10kb Optimum reaction temperature: 42°C PCR method: RT-PCR Reverse transcriptase: M-MLV Sample type: RNA Ribonuclease H activity: Low activity Product type: cDNA

Overview:

SignalChem Diagnostics Advanced Reverse Transcriptase (200 U/µL) is a modified version of the M-MLV (H-) RT, the Moloney murine leukemia virus reverse transcriptase with removed RNase activity. It offers greater cDNA synthesis efficiency, higher thermal stability, and an increased reaction temperature limit of up to 60°C.

This enzyme can synthesize cDNA products up to 10 kb in length and has improved template affinity, making it ideal for reverse transcription of RNA templates with complex secondary structures or low-copy targets.

Components:

Advanced Reverse Transcriptase (200 U/µL): 50 μL OR 5 x 50 μL
5X Advanced Buffer: 250 μL OR 5 x 250 μL

Storage and Shipping:

Store at -25°C ~ -15°C for up to 2 years. Ship on dry ice.