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SP6 RNA Polymerase

SignalChem Diagnostics SP6 RNA Polymerase (20 U/μL) is a recombinant enzyme designed for efficient in vitro transcription of RNA from DNA templates containing an SP6 promoter sequence. The enzyme utilizes NTPs as substrates and can transcribe from single- or double-stranded DNA templates, producing RNA transcripts complementary to the downstream DNA sequence. The enzyme is also able to recognize modified NTPs, such as those labelled with biotin, digoxigenin, or fluorescein, making it suitable for a broad range of molecular biology and biotechnology applications.

SR01-E21B
2 kU10 kU Bulk Customize

$ 255

*The image is illustrative and does not necessarily represent the product


Specifications:

Expression Host: Recombinant E. coli strain carrying the Salmonella typhimurium SP6 RNA gene SP6 Promoter Sequence: 5′-ATTTAGGTGACACTATAGAAGNG-3′


Storage and Stability:

Store at –25 °C ~ -15 °C for 2 years


Components:

SP6 RNA Polymerase (20 U/μL): 100 μL (2 kU); 500 μL (10 kU)
10X SP6 Reaction Buffer: 500 μL (2 kU); 2 x 1 mL (10 kU)


Additional Information:

1. Use nuclease-free pipette tips and tubes, and aliquot to avoid repeated freeze-thaw cycles.
2. For research use only.
3. Use appropriate personal protective equipment (PPE), such as a laboratory coat and gloves, and follow proper disposal protocols.




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