T7 RNA Polymerase, GMP-Grade (250 U/μL)

Specifications:

Expression Host: E. coli
Optimum Temperature: 37°C
Storage Buffer: 50 mM Tris-HCl, 1 mM EDTA, 10 mM OTT, 100 mM NaCl, 0.1% Triton X-100, 50% (v/v), glycerin, pH 7.9 at 25°C
Unit Definition: One unit is defined as the amount of enzyme required to incorporate 1 nmol of [³H]GMP into acid-insoluble precipitate in 1 hour at 37°C and pH 8.0.

Overview:

SignalChem Diagnostics T7 RNA Polymerase, GMP-Grade (250 U/μL) is a high-purity, recombinant enzyme designed for efficient in vitro transcription of RNA from DNA templates containing a T7 promoter. The enzyme utilizes NTPs as substrates and can transcribe from linear DNA templates with blunt or 5'-protruding ends, making it suitable for in vitro RNA synthesis from linearized plasmids or PCR products. Its robust performance and compliance with GMP standards make it an efficient solution for advancing RNA-based innovations and ensuring high-quality RNA production.

Storage and Shipping:

Ship on dry ice and store at -25°C ~ -15°C for up to 1 year.