T7 High-Yield RNA Synthesis Kit

Overview:

SignalChem Diagnostics T7 High-Yield RNA Synthesis Kit uses T7 RNA polymerase and a linear DNA template with the T7 promoter sequence for efficient in vitro transcription of RNA. During transcription, modified nucleotides can be added to generate biotin- or dye-labeled transcripts. The kit supports the synthesis of both short and long RNA transcripts, yielding 100–200 μg of RNA from 1 μg of DNA template. This kit can be used for RNA structure and function research, RNase protection assays, probe hybridization, RNA interference (RNAi), microinjection, and in vitro translation.

Components:

T7 RNA Polymerase Mix: 100 μL (for 50 T); 200 μL (for 100 T); 1 mL (for 500 T)
10X Transcription Buffer: 100 μL (for 50 T); 200 μL (for 100 T); 1 mL (for 500 T)
ATP (100 mM): 100 μL (for 50 T); 200 μL (for 100 T); 1 mL (for 500 T)
CTP (100 mM): 100 μL (for 50 T); 200 μL (for 100 T); 1 mL (for 500 T)
GTP (100 mM): 100 μL (for 50 T); 200 μL (for 100 T); 1 mL (for 500 T)
UTP (100 mM): 100 μL (for 50 T); 200 μL (for 50 T); 1 mL (for 500 T)
Control DNA Template (500 ng/μL): 10 μL (for 50 T); 20 μL (for 100 T); 100 μL (for 500 T)

Storage and Shipping:

Store at -25°C ~ -15°C for up to 2 years.