T7 RNA Polymerase, Low dsRNA, GMP-Grade (250 U/μL)

Specifications:

Expression Host: E. coli
Optimal Temperature: 37°C
Activity: 250 U/μL
Residual Host Nucleic Acids: <10 fg/U
Residual Host Proteins: <50 ppm
Residual RNase: Negative
Storage Buffer: 50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100, 50% (v/v) glycerol, pH 7.9 @ 25°C
Unit Definition: One unit is defined as the amount of enzyme needed to incorporate 1 nmol of [³H]GMP into an acid-insoluble precipitate in 1 hour at 37°C and pH 8.0.

Overview:

SignalChem Diagnostics T7 RNA Polymerase, Low dsRNA, GMP-Grade (250 U/μL) is a mutant of the wild-type T7 RNA Polymerase optimized for in vitro transcription (IVT) of mRNA with minimal double-stranded RNA (dsRNA) byproducts. It synthesizes RNA from double-stranded DNA templates containing a T7 promoter, using NTPs as substrates. The enzyme generates RNA complementary to the template strand downstream of the promoter.

It can use linear double-stranded DNA with either blunt ends or 5' overhangs, making it ideal for in vitro RNA synthesis from linearized plasmids or PCR products. Manufactured under Good Manufacturing Practice (GMP) standards, it guarantees batch-to-batch consistency, high purity, and regulatory compliance.

Storage and Shipping:

Store at -25°C ~ -15°C for up to one year.