mRNA Vaccinia Capping Enzyme, GMP-Grade (10 U/μL)

Specifications:

Expression Host: E. coli
Optimum Temperature: 37°C
Storage Buffer: 20 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% TritonX-100, 50% glycerin
Unit Definition: One unit is defined as the amount of enzyme needed to incorporate 10 pmol of [α-³²P]GTP into an 80-nucleotide transcript at 37°C within 1 hour.

Overview:

SignalChem Diagnostics mRNA Vaccinia Capping Enzyme, GMP-Grade (10 U/μL) is used in the capping of in vitro transcribed (IVT) mRNA to enhance its stability and translation efficiency. In eukaryotes, mRNA forms a cap structure at the 5' end after transcription, which is crucial for mRNA stability, transport, and translation. Vaccinia virus capping enzyme efficiently catalyzes cap formation and consists of two subunits, D1 and D12. It possesses RNA triphosphatase, guanylate acyltransferase, and guanine methyltransferase activities, enabling the addition of a 7-methylguanine cap (m7Gppp) to the RNA 5' end (m7Gppp5'N). Manufactured under Good Manufacturing Practice (GMP) standards, it ensures high purity, batch-to-batch consistency, and compliance with regulatory requirements.

Storage and Shipping:

Ship on dry ice and store at -25°C ~ -15°C for up to 1 year.