Glycerol-Free Enzymes

Glycerol-free enzymes are provided for applications requiring lyophilization or specialized formulation compatibility. The portfolio includes reverse transcriptases, DNA polymerases, CRISPR nucleases, RNase inhibitors, glycosidases, and uracil DNA glycosylase variants designed to maintain high activity and specificity without glycerol. Suitable for research and assay development, these enzymes support PCR, RT-PCR, CRISPR workflows, and molecular biology applications where freeze-drying or custom formulation is required.



Name Catalog # Desctiption
Advanced Reverse Transcriptase, Glycerol-Free (600 U/µL) RTH07-E29B Modified M-MLV (H-) reverse transcriptase with removed RNase activity, greater cDNA synthesis efficiency, higher thermostability, and an increased reaction temperature limit.
FnCas12a, Glycerol-Free CF12A-E311HB Recombinant full-length Francisella tularensis protein with a His tag expressed in E. coli.
Hot Start E-Taq DNA Polymerase, Glycerol-Free (5 U/μL) TQ08-E21B Hot start DNA polymerase with dual antibody-based blocking mechanism, which prevents nonspecific amplification and minimizes fluorescence signal loss.
Hot Start J-Taq DNA Polymerase, Glycerol-Free (6 U/μL) TQ09-E21B Hot start DNA polymerase that uses antibody-blocking technology to achieve high sensitivity and specificity in PCR amplifications.
LbuCas13a, Glycerol-Free CS13A-E322UB Recombinant full-length tag-free Leptotrichia buccalis LbuCas13a expressed in E. coli.
Murine RNase Inhibitor (200 U/μL), Glycerol-Free RNI05-X25A Oxidation-resistant recombinant mouse RNase inhibitor that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio with high affinity. Supplied at 200 U/μL.
Murine RNase Inhibitor (40 U/μL), Glycerol-Free RNI04-X25A Oxidation-resistant recombinant mouse RNase inhibitor that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio with high affinity. Supplied at 40 U/μL.
PNGase F, Glycerol-Free (100,000 U/mL) PNG01-E21C Amidase that efficiently cleaves N-linked glycans and is optimized for HPLC and mass spectrometry applications.
Reverse Transcriptase, High-Yield, Fast, and Thermostable, Glycerol-Free (600 U/μL) RTH05-E29B Engineered enzyme that offers high cDNA synthesis efficiency, faster reaction speed, enhanced thermal stability, and a higher reaction temperature limit. Supplied at 600 U/μL.
RNase H II, Glycerol-Free RNH01-E21C Highly thermostable ribonuclease that recognizes DNA/RNA duplexes and cleaves the RNA strand from the 5’ end, generating a 5’ phosphate and a 3’ hydroxyl group.
SpCas9-NLS, Glycerol-Free CS09N-E312HB Full-length His-tagged Streptococcus pyogenes SpCas9 protein with a nuclear localization signal (NLS).
Uracil DNA Glycosylase (UDG), Heat-Labile, Glycerol-Free UDG01-E29D Recombinant enzyme that excises uracil from DNA by cleaving N-glycosidic bonds. Active at room temperature and easily inactivated by heat.