SignalChem Diagnostics offers a comprehensive selection of high-quality molecular enzymes, specifically designed to enhance the performance and reliability of molecular diagnostic applications. Our product range includes enzymes for efficient cDNA synthesis, reliable PCR amplification, and accurate qPCR analysis. Carefully optimized and rigorously tested, these enzymes and master mixes provide consistent results, making them ideal for various molecular diagnostic assays.
| Name | Catalog # | Description |
| 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA Digester Plus) | CDN01-M29A | Ready-to-use premix with a gDNA digester, which removes residual genomic DNA contamination in RNA templates. |
| Advanced Reverse Transcriptase (200 U/µL) | RTH08-E24B | Modified M-MLV (H-) RT with removed RNase activity, greater cDNA synthesis efficiency, and higher thermostability. |
| Bst Plus DNA Polymerase | BP02-E21B | Lacks 5’-3’ exonuclease activity but has enhanced 5’-3’ DNA polymerase activity, strong strand displacement capability, and dUTP tolerance. |
| DNase I, RNase-Free | DN01-E29C | Endonuclease that degrades single-stranded and double-stranded DNA. |
| dNTP Mix (25 mM each) | DN03-M29A | Premix containing dATP, dCTP, dGTP, and dTTP, each at a concentration of 25 mM, with >99% purity (HPLC), DNase/RNase/phosphatase-free. |
| dNTP Solution Set (100 mM each) | DN02-M29A | Four separate solutions (dATP, dCTP, dUTP, and dGTP), each at a concentration of 100 mM, with >99% purity (HPLC), DNase/RNase-free. |
| Fast High-Fidelity PCR Marter Mix (With Dye) | HF02-M29A | 2X premix with High-Fidelity DNA Polymerase and electrophoresis indicators for direct loading of PCR products for electrophoresis. |
| High-Fidelity DNA Polymerase | HF01-E21B | Has both 5'→3' DNA polymerase activity and 3'→5' exonuclease activity. Its fidelity is 83 times higher than Taq DNA polymerase. |
| miRNA Universal qPCR SYBR Master Mix (Tailing) | MI01-M29A | Next-generation qPCR premix with SYBR Green I for the quantitative detection of miRNA and ROX Passive Reference Dye. |
| Multiplex One Step RT-qPCR Probe Kit (UDG Plus) | MPX02-K298 | For RNA templates with heat-resistant reverse transcriptase, hot start Taq DNA polymerase, and dUTP/UDG to prevent carryover contamination. |
| Multiplex One Step RT-qPCR Probe Kit (UDG Plus, GC Enhancer Plus) | MPX01-K298 | For RNA templates with heat-resistant reverse transcriptase for efficient first-strand cDNA synthesis and hot start Taq DNA polymerase. |
| Murine RNase Inhibitor | RNI03-X25A | Oxidation-resistant recombinant mouse RNase inhibitor that specifically inhibits RNases A, B, and C. |
| PCR Master Mix (No Dye) | TQ07-M29A | Ready-to-use 2X premix for conventional PCR. Amplified products have 3'-dA overhangs for TA cloning. |
| PCR Master Mix (With Dye) | TQ06-M29A | 2X premix for conventional PCR. Contains bromophenol blue dye for direct PCR product loading for electrophoresis. |
| Phi29 DNA Polymerase (10 U/µL) | PDP01-E24B | Phi29 DNA polymerase with exceptional strand displacement and processive synthesis activities. |
| Quick T4 DNA Ligase | T402-E24F | Designed for ligating DNA fragments and adapters during NGS library construction and validated with high-throughput sequencing. |
| Reverse Transcriptase, High-Yield, Fast, and Thermostable | RTH04-E29B | Features high cDNA synthesis efficiency, faster reaction speed, enhanced thermal stability, and a higher reaction temperature limit. |
| RNase A From Bovine Pancreas | RNA01-E27C | Endoribonuclease that specifically cleaves single-stranded RNA at pyrimidine nucleotides. |
| RNase H (5 U/μL) | RNH02-E21C | Endoribonuclease that specifically hydrolyzes phosphodiester bonds in the RNA strand of DNA/RNA hybrids. |
| RNase H II (2 U/μL) | RNH03-E21C | Highly thermostable endoribonuclease that cleaves the RNA strand of DNA/RNA hybrids and exhibits optimal activity at 70–75°C. |
| RT-LAMP Dye Assay Kit (UDG Plus) | RTL01-K298 | RT-LAMP kit with Bst Plus DNA Polymerase, Reverse Transcriptase, High-Yield, Fast, and Thermostable, and Uracil-DNA glycosylase (UDG). |
| T4 DNA Ligase | T401-E24F | Ligates blunt and sticky ends of dsDNA in the presence of ATP as a cofactor. Also repair nicks in dsDNA, dsRNA, and some DNA/RNA hybrids. |
| T7 High-Yield RNA Synthesis Kit | T702-K598 | IVT kit with T7 RNA Polymerase for short & long RNA transcript synthesis. |
| Taq DNA Polymerase | TQ05-E21B | Thermostable recombinant enzyme with 5'→3' polymerase and 5'→3' exonuclease activities but no 3'→5' exonuclease activity. |
| Ultra-Rapid Hot Start PCR Master Mix (With Dye) | HS01-M29A | Contains a modified Taq DNA polymerase, strong extension factor, amplification booster, an optimized buffer system, and a tracking dye. |
| Universal Blue qPCR Master Mix (Dye-Based) | TQ10-M29A | 2X hot start premix for real-time quantitative PCR (qPCR), blue-colored for easy sample tracking, highly sensitive and specific. |
| Universal Multiplex qPCR Master Mix (Probe-Based) | TQ11-M29A | 2x premix for qPCR that enables up to four qPCR reactions within a single reaction well. Highly sensitive and specific. Blue-colored. |
| Universal One-Step Cloning Kit | CLN02-K298 | 2X premix for homologous recombination cloning that enables direct cloning of PCR products into any vector at any desired site. |
| Universal Probe Multiplex qPCR Master Mix | TQ13-M29A | 2X premix for qPCR that supports up to four fluorescent qPCR reactions in a single well. Highly sensitive and specific. Blue-colored. |
| Universal TaqMan qPCR Mix (UDG Plus) | TQ12-M29A | Multiplex fluorescence-based qPCR premix with a next-generation, antibody-based hot start Taq polymerase. |
| Vaccinia Capping Enzyme | VC01-E54B | Catalyzes the formation of a cap structure at the 5' end of RNA. |
