Anti 2019-nCoV Spike Protein hIgG Antibody

Overview:

The severe acute respiratory syndrome related novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world in 2020 (1). The spike glycoprotein (S) of coronavirus belongs to the type I transmembrane protein containing two subunits, S1 and S2 (2), which is also known to be the key component to bind with host cells through the interaction with angiotensin-converting enzyme 2 (ACE2) (3). A receptor binding domain (RBD) of S1 can recognize the cell surface receptor and the mutation of RBD could cause higher motility rate (3).

Formulation:

PBS + 0.02% sodium azide

References:

1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3. Lan J, et al: Crystal structure of the 2019-nCov spike receptor-binding domain bound with the ACE2 receptor. bioRxiv. doi: https://doi.org/10.1101/2020.02.19.956235

Specificity:

Recognizes the 2019nCoV spike protein

Cited Applications:

ELISA, FACS

Cross Reactivity:

SARS-CoV spike protein

Host Isotype / Clone#:

Human, IgG

Stability:

1yr at –20^oC from date of shipment

Storage & Shipping:

Store product at –20^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on ice packs.

Assay Data:

Binding of anti-2019nCoV spike protein antibody (C19S1-61H) to 2019nCoV RBD (C19SD-G241H) determined by ELISA.

Assay Data:

FACS assay showed 2019-nCoV Spike protein RBD (C19SD-G241H) and 2019-nCoV Spike protein S1 (C19S1-G241F) can bind to ACE2 overexpressing cells (Panel A and B respectively). ACE2 overexpressing cells were stained with 2019-nCoV Spike protein RBD or S1, followed by anti-Spike protein antibody and fluorescence-conjugated secondary antibody.