DPP4 (29-766) Protein

Overview:

The Middle East respiratory syndrome coronavirus (MERS-CoV) has caused the MERS outbreak that first originated in Saudi Arabia, but since has spread to other countries around the world (1). The spike glycoprotein (S) of coronavirus belongs to the type I transmembrane protein containing two subunits (2), S1 and S2, which is known to be the key component to bind with host cells through interaction with dipeptidyl peptidase 4 (DPP4) (3). MERS-CoV can recognize DPP4 through its receptor binding domain (RBD) of S1 to initiate viral infection in humans (2).

Gene Aliases:

ADABP; ADCP2; CD26; DPPIV; TP103

Genebank Number:

NM_001935

Formulation:

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole.

References:

1. Mohd H, et al: Middle East Respiratory Syndrome Coronavirus (MERS-CoV) origin and animal reservoir. Viral J. 2016, 13: 87.

2. Lu G, et al: Molecular basis of binding between novel human coronavirus MERS-CoV and its receptor CD26. Nature. 2013, 500: 227-231.

3. Al-Tawfiq et al: Middle East Respiratory Syndrome-Coronavirus (MERS-CoV) Infection. Emerging Infectious Diseases. 2014, 185-190.

Activity:

For specific information on a given lot, see the related technical datasheet.

Purity:

The purity of DPP4 (29-766) was determined to be >90% by SDS-PAGE quantitative densitometry.

Storage, Stability, and Shipping:

Store product at –70^oC. For optimal storage, aliquot targets into smaller quantities after centrifugation and store at the recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

Molecular Weight:

Approx. calculated MW, 84 kDa