SARS-CoV Spike Protein S1, HIS Tag

Overview:

The severe acute respiratory syndrome related novel coronavirus (SARS-CoV) has caused the 2002-2003 SARS epidemic that broke out in southern China, which spread to other regions of Asia, Europe and North America (1). The spike glycoprotein (S) of coronavirus belongs to the type I transmembrane protein containing two subunits, S1 and S2 (2), which is also known to be the key component to bind with host cells through the interaction with angiotensin-converting enzyme 2 (ACE2). SARS-CoV can specifically recognize ACE2 through its receptor binding domain (RBD) of S1 to initiate viral infection in humans (3).

Genebank Number:

AY278554.

References:

1. Li F, et al: Receptor recognition and cross-species infections of SARS coronavirus. Antiviral Research. 2013, 100 (1): 246-254.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3. Wan Y, et al: Receptor recognition by the novel coronavirus from Wuhan: An analysis Bbsed on decade-long structural studies of SARS coronavirus. Journal of virology. 2020: 94.

Purity :

Sample Purity Data. For specific information on a given lot, see the related technical datasheet.

Storage. Stability, and Shipping:

Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight :

125 kDa

Formulations:

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole.

Assay Data:

Binding ability measured in a functional ELISA. Anti-2019-nCoV Spike Protein (C19S1-61H) binds to immobilized SARS-CoV Spike Protein S1 (His tag).

Assay Data:

Binding ability measured in a functional ELISA. Immobilized human ACE2(19-740) protein (Fc tag) (A51C2-G341F) can bind to SARS-CoV Spike Protein S1 (His tag).