Overview:

Compared to Taq DNA Polymerase, Taq Plus DNA Polymerase has higher fidelity, stronger amplification performance, and higher yield. Taq Plus Master Mix (Dye Plus) can be used for PCR amplification of animal, plant, and microbial DNA. The Master Mix can perform amplifications within 10 kb when genomic DNA is used as a template and within 15 kb when plasmid and λDNA is used as template. A protective agent in the buffer system allows 2 × Master Mix to maintain stable activity after repeated freezing and thawing. The PCR product generated has A-tailing at the 3' end and can be cloned into T vectors. A loading dye in the master mix allows the PCR products to directly be loaded for electrophoresis after the reaction. On a typical 1% agarose gel in a 1X TAE buffer or TBE buffer, the dye front migrates at the same rate as a DNA fragment of about 400 base pairs (Blue band ~400bp).

Formulation:

This master mix contains Taq Plus DNA Polymerase, dNTP, dyes and an optimized buffer system, in a proprietary formulation.