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N-Acetylneuraminic Acid Aldolase

N-Acetylneuraminic acid aldolase is a diagnostic grade reagent from a microorganism host. This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when coupled with the related enzyme in clinical analysis.

AA01D1-E311H
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Storage and Stability:

Product can be stored at 2–8°C for transportation process up to ten days but long-term storage should be at -20°C


Activity:

15 U/mg-solid or more


Unit Definition:

One unit causes the oxidation of one micromole of NADH per minute at pH 7.5 and 37°C


Grade:

Diagnostic Reagent


EC No.:

EC 4.1.3.3


Appearance:

Yellowish amorphous powder, lyophilized


Contaminants:

Catalase ≤1.0 %
NADH oxidase ≤1.0 × 10-3 %


Stabilizers:

Mannitol, EDTA


Molecular Weight:

33.9 kDa


Isoelectric Point:

6.22


Michaelis Constant:

2.8 × 10-3 M (N-Acetylneuraminic acid)


Inhibitors:

Co2+, Cu2+, Ag2+, Hg2+, NEM, Proclin


Optimum pH:

7.5 ~ 8.0


Optimum Temperature:

50°C


pH Stability:

pH 4.5 – 10.5 (25°C, 25 hr)


Thermal Stability:

Below 80°C (pH 7.5, 30 min)




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