In Vitro Transcription

In vitro transcription GMP-grade enzymes are manufactured under GMP-compliant conditions for high-purity RNA synthesis and post-transcriptional processing workflows. The portfolio includes T7 RNA polymerases, capping enzymes, methyltransferases, RNase inhibitors, DNase I, and pyrophosphatases designed to support efficient transcription, capping, DNA removal, and RNA stabilization. These enzymes enable consistent, high-quality mRNA production for advanced research and biopharmaceutical applications.



Name Catalog # Description
DNase I, GMP-Grade (2 U/μL) DE01-E59C Endonuclease that digests both ssDNA and dsDNA, used to remove DNA contaminants from RNA and protein samples for optimal purity in downstream applications.
Inorganic Pyrophosphatase, GMP-Grade (0.1 U/μL) PYR01-E59C Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) into orthophosphate (Pi), can be used to remove PPi and improve RNA yield. Supplied at 0.1 U/μL.
Inorganic Pyrophosphatase, GMP-Grade (1 U/μL) PYR02-E59C Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) into orthophosphate (Pi), can be used to remove PPi and improve RNA yield. Supplied at 1 U/μL.
mRNA Cap 2’-O-Methyltransferase, GMP-Grade (50 U/μL) MET01-E59B Catalyzes the formation of the Cap1 structure, which enhances mRNA translation efficiency.
mRNA Vaccinia Capping Enzyme, GMP-Grade (10 U/μL) VAC01-E59B Catalyzes the formation of a cap structure at the 5' end of in vitro transcribed mRNA, used to enhance its stability and translation efficiency.
Murine RNase Inhibitor, GMP-Grade (40 U/μL) RNI02-X55A Oxidation-resistant recombinant mouse RNase inhibitor that specifically inhibits RNases A, B, and C.
RNase R, GMP-Grade (20 U/μL) RNR01-E59C Magnesium-dependent 3' → 5' exoribonuclease that selectively degrades linear RNA but does not degrade circular and highly structured RNAs.
T7 RNA Polymerase, GMP-Grade (250 U/μL) T702-E51B Designed for efficient in vitro transcription of RNA from DNA templates containing a T7 promoter. Supplied at 250 U/μL.
T7 RNA Polymerase, GMP-Grade (50 U/μL) T701-E51B Designed for efficient in vitro transcription of RNA from DNA templates containing a T7 promoter. Supplied at 50 U/μL.
T7 RNA Polymerase, Low dsRNA, GMP-Grade (250 U/μL) T703-E51B Engineered T7 RNA Polymerase optimized for in vitro transcription of mRNA with minimal dsRNA byproducts and used for RNA synthesis from linearized plasmids or PCR products.