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Creatine Amidinohydrolase

This enzyme is a diagnostic-grade reagent. It is used for enzymatic determination of creatine and creatinine in clinical analysis when coupled with creatinine amidohydrolase and sarcosine oxidase.

CR02D1-E311H
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Storage and Stability:

Product can be stored at 2~8°C for transportation process up to ten days but long-term storage should be at –20°C


Activity:

≥ 4 U/mg solid


Unit Definition:

One unit is defined as the amount of enzyme that produces 1 μmol of urea per minute at 37°C and pH 7.7 under the conditions described below.


EC No.:

EC 3.5.3.3


Applications:

It is used for enzymatic determination of creatine and creatinine in clinical analysis when coupled with creatinine amidohydrolase and sarcosine oxidase.


Grade:

Diagnostic Reagent


Appearance:

White amorphous powder, lyophilized


Contaminants:

Catalase ≤ 2.0 %
NADH oxidase ≤ 5.0 ×10-2 %


Stabilizers:

Sugars, EDTA


Molecular Weight:

47 kDa


Isoelectric Point:

5.6


Michaelis Constant:

6.7 × 10⁻² M (creatine)


6.7 × 10⁻² M (creatine):

Cu2+, Hg2+, Ag+, Zn2+, IAA, NEM, ProClin-300


Optimum pH:

8.0


Optimum Temperature:

30°C


pH Stability:

pH 6.0–8.0 (25°C, 24 hr)


Thermal Stability:

Below 55°C (pH 7.5, 30 min)




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