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Cholesterol Oxidase

Cholesterol oxidase is a recombinant diagnostic grade reagent from a microorganism host. This enzyme is useful for enzymatic determination of cholesterol in serum when coupled with cholesterol in clinical analysis.

CX01D1-E311H
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Storage and Stability:

Product can be stored at 2–8°C for transportation process up to ten days but long-term storage should be at -20°C


Activity:

≥ 12 U/mg solid


Unit Definition:

One unit is defined as the amount of enzyme which liberates 1 μmol of △4-Cholest-3-one per minute at 37°C at pH 7.0.


Grade:

Diagnostic Reagent


EC No.:

EC 1.1.3.6


Application:

This enzyme is used for enzymatic determination of cholesterol in serum when coupled with cholesterol in clinical analysis.


Appearance:

Yellowish amorphous powder, lyophilized


Contaminants:

Catalase ≤1.0 × 10-1 %
Cholesterol esterase ≤1.0 ×10-2 %


Stabilizers:

BSA, amino acids


Molecular Weight:

60 kDa


Isoelectric Point:

8.4


Michaelis Constant:

1.2 ×10-5 M (Cholesterol)


Inhibitors:

Cu2+, Ag+, Hg2+


Optimum pH:

6.5 ~ 7.2


Optimum Temperature:

55°C


pH Stability:

pH 5.5–10.5 (25°C, 20 hr)


Thermal Stability:

Below 50°C (pH 7.0, 15 min)




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