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Diaphorase

This enzyme is a diagnostic grade reagent from a microorganism host. It is useful for enzymatic determination of reduced NAD.

DA01D1-E311H
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Storage and Stability:

Product can be stored at 2~8°C for transportation process up to ten days but long-term storage should be at –20°C.


Activity:

100U/mg-solid or more


Unit Definition:

One unit causes the decrease of one micromole of DCPIP per minute at 37°C under the conditions specified in the assay procedure.


Applications:

It is useful for enzymatic determination of reduced NAD.


EC No.:

EC 1.6.5.2


Grade:

Diagnostic Reagent


Appearance:

Yellowish amorphous powder, lyophilized


Contaminants:

Myokinase ≤5.0 ×10-1 %
NADH oxidase ≤1.0 × 10-1 %


Molecular Weight:

26.3 kDa


Isoelectric Point:

7.72


Michaelis Constant:

2.23 ×10-1 M (NADH), 5.72 ×10-1 M (NADPH)


Inhibitors:

Fe3+, Cu2+, Hg2+, Ag+


Optimum pH:

6.0, 8.0-10.0


Optimum Temperature:

40~45°C


pH Stability:

pH 6.0~11.0 (25°C, 20 hr)


Thermal Stability:

below 62°C (pH 7.5, 15 min)




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