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DNA Library Prep Kit (Mechanical)

SignalChem Diagnostics DNA Library Prep Kit (Mechanical) is designed to generate high-quality DNA sequencing libraries for Illumina™ and MGI™ platforms. The kit is optimized to have high efficiency in end repair, dA-tailing, and adapter ligation. It supports a broad DNA input range from 100 pg to 1 μg and is compatible with a diverse range of DNA sample sources, including genomic DNA, FFPE tissue, circulating cell-free DNA (cfDNA), and ChIP-derived DNA.

Amplification is driven by a high-fidelity DNA polymerase that provides high accuracy and uniformity and is designed to enhance detection of low-frequency variants. This kit uses an engineered ligase to maximize adapter ligation efficiency while reducing formation of dimers, resulting in higher library conversion rates and improved sequencing quality.

DLP03-K298
24 T96 T Bulk Customize

$ 580

*The image is illustrative and does not necessarily represent the product


Components:

Buffer (green label): 168 μL (24 T); 672 μL (96 T)
Enzyme (yellow label): 72 μL (24 T); 288 μL (96 T)
Ligation Enhancer (red label): 720 μL (24 T); 3 x 960 μL (96 T)
T4 DNA Ligase (blue label): 240 μL (24 T); 2 x 480 μL (96 T)
Amplification Mix (white label): 600 μL (24 T); 3 x 800 μL (96 T)
Note: The kit components are compatible with both Illumina and MGI sequencing platforms; however, if using a complete adapter, additional primer mix (not included in this kit) is required.


Storage and Shipping:

Store at –25 °C ~ -15 °C for 1 year


Additional Information :

1. Thaw all components at room temperature. Once thawed, mix thoroughly by vortexing, briefly spin down, and place on ice until use.
2. When preparing reaction solutions, mix gently using a pipette or by lightly shaking. Avoid vigorous shaking, as it may reduce library yield.
3. Always use filtered pipette tips to prevent cross-contamination.
4. For research use only.




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