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One-Pot DNA Library Prep Kit (Enzymatic)

SignalChem Diagnostics One-Pot DNA Library Prep Kit is an advanced one-tube solution designed for DNA fragmentation and library preparation, compatible with both Illumina™ and MGI™ sequencing platforms. Unlike other commercial kits that use enzyme-based fragmentation, this optimized kit minimizes bias and artifacts while efficiently generating libraries from as little as 100 pg up to 1000 ng of DNA.

By combining fragmentation and end-repair into a single reaction, it streamlines the workflow, making library preparation faster and easier. Additionally, the ligation module comes with premixed enzymes and a buffer, significantly reducing both time and cost, making it ideal for automated library construction.

This kit offers a high library conversion rate and is compatible with DNA from a wide range of sources, including animals, plants, microorganisms, and FFPE samples. Its high-fidelity enzyme enhances amplification accuracy and ensures uniform coverage, delivering reliable sequencing results.

DLP04-K298
8 T24 T96 T Bulk Customize

$ 298

*The image is illustrative and does not necessarily represent the product


Storage and Stability:

Store at –25 °C ~ -15 °C for 1 year


Components:

Buffer (yellow label): 80 μL (8T); 240 μL (24T); 960 μL (96T);
Enzyme (yellow label): 80 μL (8T); 240 μL (24T); 960 μL (96T);
Ligation Enhancer (blue label): 240 μL (8T); 720 μL (24T); 3 x 960 μL (96T);
Rapid DNA Ligase (blue label): 80 μL (8T); 240 μL (24T); 2 x 480 μL (96T);
2X Amplification Mix (white label): 200 μL (8T); 600 μL (24T); 3 x 800 μL (96T);

Note: The kit components are compatible with both Illumina and MGI sequencing platforms; however, if using a complete adapter, additional primer mix (not included in this kit) is required.


Additional Information:

1. Thaw all components at room temperature. Once thawed, mix thoroughly by vortexing, briefly spin down, and place on ice until use.
2. When preparing reaction solutions, mix gently using a pipette or by lightly shaking. Avoid vigorous shaking, as it may reduce library yield.
3. Always use filtered pipette tips to prevent cross-contamination.
4. For research use only.




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