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DNA Topoisomerase I (Scl-70, full length) Antigen

Recombinant Human DNA Topoisomerase I (Scl-70; full length) Antigen was expressed by baculovirus in Sf9 insect cells and purified by affinity chromatography using a His tag.

DTA01-F23H
100 µg1 mg Bulk Customize

$ 260

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Alternate Names:

Cyclin, DNA topoisomerase


Formulation:

Protein stored in 16 mM HEPES, pH 7.5, 400 mM NaCl, 20% Glycerol


References:

1. Meesters, et al. Analysis of an immunodominant epitope of topoisomerase I in patients with systemic sclerosis. Mol Biol Rep 16, 117–123 (1992).

2. Verheijen R, et al. A recombinant topoisomerase I used for autoantibody detection in sera from patients with systemic sclerosis. Clin Exp Immunol. 1990 Apr;80(1):38-43.

3. Czömpöly T, er al. Anti-topoisomerase I autoantibodies in systemic sclerosis. Autoimmunity Reviews, 2009, 8(8): 692-696.

Storage and Stability:

Store at –70°C. To avoid repeated handling and multiple freeze/thaw cycles, aliquot product into smaller quantities


Source:

Homo Sapiens (Human)


Host:

Baculovirus-infected Sf9 insect cells


Molecular weight:

Calculated: 102 kDa
Observed: ~102 kDa


Purity:

The purity was determined to be ≥ 85% by densitometry




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