Home   >   Reagents   >    Clinical Chemistry    >    Diagnostic-Grade Enzymes

Signalchem Product Image

Glucose-6-phosphate dehydrogenase

Glucose-6-phosphate dehydrogenase is a diagnostic grade reagent from a microorganism host. This enzyme is useful for enzymatic determination of NAD+ (NADP+) and G-6-P, and activities of phosphoglucose isomerase, phosphoglucomutase, and hexokinase. The enzyme is also used for enzymatic determination of glucose and creatine phosphokinase activity when coupled with hexokinase.

GD03D1-E311H
20 kU50 kU100 kU200 kU Bulk

$ 0

*The image is illustrative and does not necessarily represent the product


Storage and Stability:

Product can be stored at 2–8°C for transportation process up to ten days but long-term storage should be at -20°C


Activity:

280U/mg-solid or more


Unit Definition:

One unit causes the formation of one micromole of NADH per minute at pH 7.8 at 30°C


Grade:

Diagnostic Reagent


EC No.:

EC 1.1.1.49


Appearance:

White amorphous powder, lyophilized


Contaminants:

Creatine phosphokinase & Phosphoglucomutase all ≤1.0 ×10-3 %
6-Phosphogluconate dehydrogenase ≤5.0 ×10-3 %
Phosphoglucose isomerase ≤1.0 × 10-2 %
Glutathione reductase ≤1.0 × 10-3 %
Hexokinase & Myokinase all ≤1.0 ×10-2 %
NADH oxidase & NADPH oxidase all ≤1.0 ×10-2 %


Molecular Weight:

55 kDa


Isoelectric Point:

5.1


Michaelis Constant:

1.1×10-4 M (G6P), 2.0 ×10-4 M (NAD+)


Inhibitors:

Fe3+, Ni2+, Hg2+, SDS


Optimum pH:

7.0 ~ 8.0


Optimum Temperature:

55°C


pH Stability:

pH 4.4–10.5 (25°C, 20 hr)


Thermal Stability:

Below 45°C (pH 7.5, 15 min)




There are no related publications available for this product.