Home   >   Reagents   >    Clinical Chemistry    >    Diagnostic-Grade Enzymes

Signalchem Product Image

Glutamate dehydrogenase (NAD-dependent)

This enzyme is a diagnostic grade reagent from a microorganism host. It is useful for enzymatic determination of NH3, α-Ketoglutaic acid and L-Glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease in clinical analysis.

GD04D1-E311H
Bulk

$ 0

*The image is illustrative and does not necessarily represent the product


Storage and Stability:

Product can be stored at 2~8°C for transportation process up to ten days but long-term storage should be at –20°C.


Activity:

80 U/mg-solid or more


Unit Definition:

One unit produces one micromole of NAD+ per minute at pH 7.3 and 25°C


Grade:

Diagnostic Reagent


EC No.:

EC 1.4.1.2


Applications:

This enzyme is useful for enzymatic determination of NH3, α-Ketoglutaic acid and L-Glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease in clinical analysis.


Appearance:

White amorphous powder, lyophilized


Contaminants:

NAD oxidase ≤1.0 × 10-2 %


Molecular Weight:

47.3 kDa


Michaelis Constant:

5.4 ×10-2 M (NH3), 7.83 ×10-5 M (α-Ketoglutarate), 3.0 ×10-3 M (L-Glutamate), 4.13 × 10-5 M (NADH), 3.16 ×10-3 M (NAD+)


Inhibitors:

Heavy metals, SDS


Optimum pH:

8.0 – 8.5


Optimum Temperature:

65°C


pH Stability:

pH 5.0–10.0 (25°C, 20 hr)


Thermal Stability:

Below 55°C (pH 7.3, 10 min)




There are no related publications available for this product.