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Glutamate Decarboxylase 65kDa (GAD 65) Antigen

Recombinant Human Glutamate Decarboxylase 65 kDa (GAD65) Antigen was expressed by baculovirus in Sf9 insect cells and purified by affinity chromatography using His tag.

GDA01-F23H
100 µg1 mg Bulk Customize

$ 260

*The image is illustrative and does not necessarily represent the product


Alternate Names:

65 kDa glutamic acid decarboxylase, Islet Cell Autoantigen GAD65


Formulation:

Protein stored in 16 mM HEPES, pH 7.5, 250 mM NaCl, 0.016 mM Pyridoxal-5-Phosphate, 0.16% Triton X-100, 20% Glycerol


References:

1. Baekkeskov S, et al. Identification of the 64K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylase. Nature. 1990 Sep 13;347(6289):151-6.

2. Walikonis JE, et al. Radioimmunoassay for glutamic acid decarboxylase (GAD65) autoantibodies as a diagnostic aid for stiff-man syndrome and a correlate of susceptibility to type 1 diabetes mellitus. Mayo Clin Proc. 1998 Dec;73(12):1161-6.

3. Towns R, et al. GAD65 autoantibodies and its role as biomarker of Type 1 diabetes and Latent Autoimmune Diabetes in Adults (LADA). Drugs Future. 2011 Nov;36(11):847.

Storage and Stability:

Store at –70°C. To avoid repeated handling and multiple freeze/thaw cycles, aliquot product into smaller quantities


Source:

Homo Sapiens (Human)


Host:

Baculovirus-infected Sf9 insect cells


Molecular Weight:

Calculated: 64 kDa
Observed: ~65 kDa


Purity:

The purity was determined to be ≥ 85% by densitometry




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