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Glucose Oxidase

This enzyme is a diagnostic grade reagent from a microorganism host. It is useful for enzymatic determination of glucose, and for amylase-activity assay when coupled with α-glucosidase (if maltooligosaccharide or modified starch is used as a substrate) in clinical analysis.

GX01D1-E311H
20 kU50 kU100 kU200 kU Bulk

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Storage and Stability:

Product can be stored at 2–8°C for transportation process up to ten days but long-term storage should be at -20°C


Activity:

70 U/mg-solid or more


Unit Definition:

One unit causes the formation of one micromole of hydrogen peroxide (half a micromole of quinoneimine dye) per minute at 37°C.


Grade:

Diagnostic Reagent


EC No.:

EC 1.1.3.4


Appearance:

Grey green amorphous powder, lyophilized


Contaminants:

Catalase ≤ 3.0 %


Stabilizers:

Potassium gluconate, sodium glutamate


Molecular Weight:

65 kDa


Isoelectric Point:

5.43


Michaelis Constant:

2.54 ×10-2 M (β-D-Glucose)
4.15 ×10-2 M (2-Deoxyglucose)


Inhibitors:

Cu2+, Ag+, Hg2+, Fe3+


Optimum pH:

4.5 ~ 5.5


Optimum Temperature:

45 ~ 50°C


pH Stability:

pH 4.5 – 9.0 (30°C, 20 hr)


Thermal Stability:

Below 55°C (pH 5.7, 1 hr)




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