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D-3-Hydroxybutyrate Dehydrogenase

This enzyme is a diagnostic grade reagent from a microorganism host. It is useful for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.

HD01D1-E311H
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Storage and Stability:

Product can be stored at 2~8°C for transportation process up to ten days but long-term storage should be at –20°C.


Activity:

50U/mg-solid or more (Grade III)


Unit Definition:

One unit causes the formation of one micromole of NADH per minute at pH 8.5 at 37°C


Applications:

It is useful for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.


EC No.:

EC 1.1.1.30


Grade:

Diagnostic Reagent


Appearance:

White amorphous powder, lyophilized


Contaminants:

Malate dehydrogenase, Lactate dehydrogenase and NADH oxidase all ≤2.0 ×10-3 %


Stabilizers:

Sucrose, mannitol, BSA


Molecular Weight:

28 kDa


Isoelectric Point:

6.59


Michaelis Constant:

3.79 ×10-3 M (37°C), 2.04 ×10-3 M (25°C) pH 8.5 (D-3-Hydroxybutyrate)
7.74 ×10-5 M (37°C), 3.68 ×10-5 M (25°C) pH 8.5 (NAD+)


Inhibitors:

PCMB, IAA, MIA, Cu2+, Hg2+, Ag+, NEM, EDTA, SDS, Procline


Optimum pH:

7.5-8.5


Optimum Temperature:

55°C


pH Stability:

pH 5.5~8.0 (25°C, 24 hr)


Thermal Stability:

Below 45°C (pH 8.5, 30 min)




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