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Hexokinase

Hexokinase is a diagnostic grade reagent from a microorganism host. This enzyme is useful for enzymatic determination of glucose, adenosine-5’-triphosphate (ATP) and creatine phosphokinase when coupled with glucose-6-phosphate dehydrogenase.

HK01D1-E311H
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Storage and Stability:

Product can be stored at 2–8°C for transportation process up to ten days but long-term storage should be at -20°C


Activity:

150U/mg-solid or more


Unit Definition:

One unit causes the formation of one micromole of NADH per minute at pH 8.0 at 30°C.


Grade:

Diagnostic Reagent


EC No.:

EC 2.7.1.1


Appearance:

White amorphous powder, lyophilized


Contaminants:

Phosphoglucose isomerase ≤1.0 × 10-1 %
6-Phosphogluconate dehydrogenase ≤1.0 × 10-2 %
Glucose-6-phosphate dehydrogenase ≤1.0 × 10-2 %
Myokinase ≤1.0 × 10-2 %
Glutathione reductase ≤5.0 × 10-1 %


Molecular Weight:

55.1 kDa


Isoelectric Point:

5.8


Michaelis Constant:

6.1×10-5M (D-Glucose), 9.6 ×10-5 M (ATP)


Inhibitors:

Co2+, Fe3+, Ag+, Hg2+, SDS


Optimum pH:

8.0 ~ 9.0


Optimum Temperature:

40 ~ 50°C


pH Stability:

pH 5.0–9.5 (25°C, 20 hr)


Thermal Stability:

Below 40°C (pH 7.5, 15 min)




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